Cobra Effect · The brain

Is the brain one net or many cells?

How a black stain and a famous feud revealed that the brain is made of separate cells.

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A microscope beside a glass slide with one dark branching nerve cell on it

In 1873, Camillo Golgi found a way to stain nerve cells black.

Golgi was an Italian doctor, working at a hospital for the chronically ill. He hardened nerve tissue in one chemical, then soaked it in silver nitrate. Only a few cells turned black, but each one showed completely, down to its finest branches. He called it the black reaction.

Golgi believed the nerve fibres fused together into one continuous net.

Under his microscope, the finest branches seemed to run into one another. He pictured the nervous system as a single joined network. Many scientists agreed with him.

In Spain, Santiago Ramon y Cajal took up Golgi’s stain.

Cajal had wanted to be an artist as a boy, and he drew what he saw with great care. In 1887 he saw slides made with Golgi’s method, and set about improving it. Tracing branch after branch, often in birds and young animals, he followed each one to its end. The branches came close to other cells, but did not join them.

In 1888, Cajal argued that the nervous system is made of separate cells.

Each cell, he said, is a distinct unit, touching others but not fused with them. In 1891 the German anatomist Wilhelm Waldeyer called these cells neurons. The idea became known as the neuron doctrine.

In 1906, Golgi and Cajal shared the Nobel Prize, and used their lectures to disagree.

They were honoured together for their work on the structure of the nervous system. Golgi spoke first, and said he had always been opposed to the neuron theory. The next day, Cajal described nerve cells meeting by contact, not by continuity. Golgi never changed his mind.

In the 1950s, electron microscopes finally settled the argument.

They could show far finer detail than any light microscope. They revealed tiny gaps between nerve cells, where signals pass from one to the next. Cajal’s picture had won. Even so, a few nerve cells are directly joined, just as Golgi might have liked.

So when two experts see the same evidence differently, ask what finer detail could decide it.

Golgi and Cajal used the same stain and saw different things. Their argument could not be settled until a sharper instrument existed. Sometimes the honest answer is to keep looking, and wait for better evidence.

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